06. Detection of virus and bacterial contaminants of semen of pedigree bulls with the use of molecular-genetic methods

Stegnij B. T., Gerilovych A. P., Bolotin V. I., Isakov M. M., Baschenko M. I.
Pages: 27-31.

Full article: 
Abstract
The purpose. To tap contamination of deep-frozen semen of pedigree bulls with virus and bacterial agents. Methods. Bacteriological, molecular-genetic, statistical. Results. 826 samples from 6 areas of Ukraine are examined. That has allowed tapping genetic material of virus.es of contagious rhinotracheitis and diarrhea of cattle in 3,5 and 6, 1 % of events accordingly. DNA of Chlamydia and mycoplasma was taped in 3, 15 and 8,6% of the examined samples accordingly. The belonging of virus of the diarrhea found in semen of bulls to 2 genotype of the causal organism is fixed Conclusions. Considering existing risks of spread of communicable diseases through contaminated semen of bulls, there is a necessity of implementing measures on profound quality control of genetic resources of cattle.

Key words: liquefrozen semen, bulls, virus and bacte­rial contamination, polymerase chain reaction, labora­tory diagnosis.

References
  1. Veterinary and sanitary requirements for semen in artificial insemination of animals/N.G. Balashov, AK Butakov, VN Rodina, NF Chuklov - M .: Kolos, 1977. - 220 p.
  2. Infectious rhinotracheitis of cattle/AG. Glotov, AF Shulyak, GI Glotov, AN Sergeev//RASHN, Sib. Otd-hye, GHY YEBCyDB. - Novosibirsk: IPI "Jupiter", 2006, - P. 196.
  3. Polymerase chain reaction in the practice of veterinary medicine: science-method. manual/B.T. Stegany, AP Gerylovich, O.Yu. Liman and others; under ed. B.T. Stegnia and AP Gerylovich. - Kh .: NSC "IKEVM", 2006. - 110 p.
  4. Development of duplex PCR for indicating mycoplasmas and bovine diarrhea viruses, followed by genotyping of the pathogen/AP. Gerylovich, IV Gorayichuk, VI Bolotin, O.S. Solodiankin//Visn. Ukr t-va geneticists and breeders. - Lviv, 2013. - Vol. 11, No. 1. - P. 28 - 33.
  5. Farbotko G. E. Detection of viruses in semen of bulls/GE. Farbotko, NN Kondrachin//Veterinary Medicine. - 1992. - No. 5. - P. 47-48.
  6. Analytical sensitivity of the assays used to detect bovine virus in the diarrhea virus in semen samples from the Southeastern United States/M.D. Givens, A.M. Heath, R.L. Carson et al .//Vet Microbiol. - 2003. - V. 96 (2). - P. 145-155.
  7. Bolin S.R. Control of bovine virus diarrhoea virus/S.R. Bolin//Res. Sci Tech - 1990. - V. 9, No. 1. - P. 163 - 171.
  8. Comparison of the bacteriological properties of various egg yolk sources and in vitro and in vivo fertilization potential of bovine semen frozen in egg yolk or lecithin based diluents/S. Bousseau, J.P. Brillard, B. Marguant-Le Guienne et al.//Thyriogenology. - 1998. - V. 50 (5). - P. 699 - 706.
  9. Comparison of three polymerase chain reaction methods for routine detection of bovine herpesvirus 1 DNA in fresh bull sperm/C. B. Smits, C. Van Maanen, R.D. Glas et al .//Virol Methods. - 2000. - V. 85 (1 - 2). - P. 65 - 73.
  10. Detection of bovine viral diarrhea virus in sperm obtained after inoculation of seronegative postpubertal bulls/M.D. Givens, A.M. Heath, K.V. Brock, B.W. Brodersen//Vet Res. - 2003. - V. 64 (4). - P. 428 - 434.
  11. Isolation of an avian serovar of Chlamydia psittaci from a case of bovine abortion/H.U. Cox, P.G. Hoyt, R.P. Poston et al.//Vet Diagn Invest. - 1998. - V. 10. - P. 280-228.
  12. PCR-screening of Chlamydiae and Viral Contamination of Bull Semen in Farms of Southern and Eastern Ukraine/A.P. Gerilovych, A.V. Skrypnyk, V.I. Bolotin et al .//Infektionen durch intrazellulare Erreger-Diagnostika, Epidemiologie und Pathgenese der Chlamydien- und Coxielleninfektionen: Zusammenarbeit mit dem NRL Q-Fieber und dem AVID (Jena, 25-26 September 2008). - Yena, 2008. - P. 39 - 41.
  13. Potential risk of BLV distribution via bulls sperm/D. Beier, K. Bogner, A. Krisch et al.///Proc. European virology association congress. - 2011. - P. 113.
  14. Prevalence of chlamydiae in semen and genital tracts of bulls, rams and bucks/K. Teankum, A. Pospischil, F. Janett et al .//Theriogenology. - 2007. - P.
  15. - P. 303-310. 15. Rapid detection of bovine herpesvirus in the semen of infected bulls by a nested polymerase chain reaction assay/S.A. Masri, W. Olson, P.T. Nguyen et al.//J. J. Vet Res. - 1996. - V. 60 (2). - P. 100 - 107.
  16. Ureaplasma diversum as a cause of reproductive disease in cattle/B. Miller, A. Chelmonsky, B. Smits et al.//Food Animal Practice. - 1994. - V. 10. - P. 479-490.
  17. Virulence and genotype of a bovine herpesvirus 1 isolate from a semen of a subclinically infected bull/J.T. van Oirschot, F.A. Rijsewijk, P.J. Straver et al .//Vet Res. - 1995. - V. 137 (10). - P. 235-239.