06. Molecular-genetic and phylogenetic researches of virus of leukosis of cattle circulating in Ukraine

Baschenko M. I., Stegnij B. T., Gerilovych A. P., Lymans'ka O. Ju., Gema I. O., Gorbatenko S. K.
Pages: 30-33.

Full article: 
Abstract
The purpose. To compare the effectiveness of the application of molecular genetic methods and RID in the diagnosis of bovine leukemia and to investigate the phylogenetic relationships between isolates of the leukemia virus (VL) of cattle. Methods. Detection of the pro-viral DNA of VL of cattle was carried out using the domestic PCR test system "BLV-provirus OL / L-hesg", developed by the NSC "IEKVM". Results. The molecular genetic features and phylogenetic connections in the population of HL circulating in different regions of Ukraine are researched. The higher specificity of PCR analysis in comparison with RID for detecting HL of the cattle is confirmed. Conclusions. It has been established that the results of phylogenetic research can be used to identify and study possible subgroups (or genotypes), to create a basis for the search for genes that determine the high biological activity of viruses.

Key words: virus, leukosis or cattle, molecular-genetic methods, phylogenetic analysis.

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