08. Identification sporeforming bacteria in associations of hydrogen-generating microorganisms

Tashyrev O. B., Prytula I. R., Tashyreva G. O.
Pages: 41-46.

Full article: 
Abstract
The purpose. To determine species composition of spore-forming bacteria of associations of hydrogen-generating microorganisms for assessment of an opportunity of their biotechnological application. Methods. Phonotypical and phylogenetic analysis, chemical­alytical, gas chromatography. Results. Bacillus and Clostridium predominate in structure of an probed associations. Under morphological, cultural-biochemical and molecular-genetic characteristics they identified 3 strains to species. The strain of anaerobic bacteria is identified as Clostridium butyricum VU-11. strains of aerobic bacteria - as Bacillus licheniformis BS-3 and Bacillus atrophaeus BS-1. Conclusions. Bacillic-clostridial associations are perspective for cost effective synthesis or power supply H2 from a wide spectrum of renewable biomass, including food waste.


Key words: hydrogen, associations of hydrogen-generating microorganisms, identification, Clostridium, Bacillus.



References
  1. Matveeva N.A. Formation of molecular hydrogen by the association of spore-forming microorganisms/N.A. Matveyev [and others.]//Microbial. Journ - 2011. - T. 73, No. 1. - P. 36-43.
  2. Methods of general bacteriology: in 3 tons/[ed. F. Gerhard [and others]. - M .: Mir, 1984. - T. 3. - 263 p.
  3. Pritula I.R. Application of redox indicators for measuring the oxide-to-new potential during growth of microorganisms cultures/I.P. Prytula, OB, Tashirova//Biological Studies/ZSsiaia Vioiodis. - 2013. - T. 7, No. 3, -S. 133-144
  4. Pritula I.R. Improvement of the method of separation of hydrogen-forming bacteria of the genus SIOosShiItM.R. Prytula, AB Tashirov//Microbial. Journ - 2012. - Vol. 74, No. 6. - P. 58-64.
  5. Romanovskaya V.O. Phylogenetic analysis of bacteria of extreme ecosystems/VO Romanovskaya and others.//1U.Proboyul. Journ - 2014. - Vol. 76, No. 3. - P. 2-10.
  6. Choi J. Characteristics of biohydrogen fermentation from various substrates/J. Choi, Y. Ahn//lnt. J. Hydrog. Energy - 2014. - V. 39, No. 7. - P. 3152-3159.
  7. Das D. Advances in biological hydrogen production processes/D. Das, T. N. Veziroglu//Int. J. Hydrog. Energy - 2008. - V. 33. - P. 6046-6057.
  8. De Vos P. Bergey's Manual for systematic bacteriology/P. De Vos [and others] - New York, Springer. - 2009. - V. 3, 2d ed.
  9. Liu l.-C. The effect of pH on the production of biohydrogen by clostridia: thermodynamic and metabolic considerations/l.-C. Liu [et al.]//Int. J. Hydrog. Energy - 2011, - V. 36, No. 1. - P. 439-449.
  10. Meade H.M. Physical and genetic cha-racterization of symbiotic and auxotrophic mutations of Rhizobium meiiioti induced by tranposon Tn5 mutagenesis/H.M. Meade [et al.]//J. Bacteriol - 1982. - V. 149, No. 1. - P. 114-122.
  11. Miniprep of bacterial genomic DNA. Current protocols in molecular biology [Electronic resource]: Access mode: http://www.falw.vu/~microb/Protocols/nucleic_acid_ isolation/DNA_isolation_Bacteria.pdf
  12. Ntaikou I. Biohydrogen production from biomass and waste through dark fermentation: a Review/l Ntaikou, G. Antonopoulou, G. Lyberato//Waste Biomass Valor. - 2010, - V. 1, -P. 21-39.
  13. Reva O.N. Simplified technique for the identification of aerobic spore-forming bacteria by phenotype/O.N. Reva I.B. Sorokulova, V.V. Smirnov//lnt. J. Syst. Evolut Microbiol - 2001. - V. 51, №4. -P 1361- 1371.
  14. Tashyreva A. The novel comprehensive approach for non-food agricultural and landfill biomass microbial fermentation and biogas production. Tashyreva, O. Tashyrev, I. Prytula//Biotechnology and Plant Breeding Perspectives; Eds R.K. Behl, E. Arseniuk//Agrobios (International) Publishers, 2014. - pp. 337- 346.