08. DNA-certification of breeds of bees of Ukraine in the system of preservation and development of their gene pool

Metlyts'ka O. I., Kovtun S. I., Pal'kina M. D.
Pages: 39-43.

Full article: 
Abstract
Goal. To develop a method of DNA passporting and to establish gene pool standards for purebredness in domestic beekeeping by molecular genetic fingerprint markers with multiple localization in the genome (RAPD, ISSR). Methods. Molecular genetic, statistical, morphometric, ethological. Results The results of molecular-genetic monitoring of bees of Ukrainian, Carpathian and gray mountain Caucasian breeds for using different technologies of detection of polymorphism of genetic loci are presented. Genetic passports of rocks were developed based on identified identification alleles. The absolute marker of the gray mountainous Caucasian breed G800, which was present in all its representatives, is a reliable tool for identifying families of indigenous origin of bees of Ukrainian aboriginal breeds. Conclusions Establishing genetically determined genetic differences, confirmed by genetic-population indicators, makes it possible to create natural reserves of pure-breed bees as a compulsory component in the system of preservation of their gene pool and breeding improvement.

Key words: identification marker, genetic passport, bees breed, purebred criterion, gene pool.

References
  1. Ydehtyfykatsyia nopod y nonyliatsyj medohochoj nchelbl c ycnolbzobahyem metoda PTsP/ H.Y. Kpybtsob, H.Y. Gopiachoba, Y.G. Ydyha y dp.//Celbckoxoziajctbehhaia byoloryia. – 2010. – № 6. – C. 26 — 29.
  2. Ylbiacob P.A. Polymopfyzm Apis mellifera mellifera L. ha Ypale: abtopef. dycc. ha coyckahye ych. cten. kahd. byol. hayk: cnets. 03.00.15. – Gehetyka/P.A. Ylbiacob. – Yfa, 2006. – 20 c.
  3. Kalehdapb P.H. Komnbiutephaia nporpamma dlia noctpoehyia eboliutsyohhblx depebbeb ha ochobe elektpofoperpamm DHK y belkob/P.H. Kalehdapb// Matepyalbl kohf. «Molekyliapho-rehetycheckye mapkepbl y celektsyia pactehyj». – K., 1994. – C. 25 — 26.
  4. Kpybtsob H.Y. Pposhloe, hactoiaschee y bydysche nchelobodctba/H.Y. Kpybtsob//Zootexhyia. – 2008. –№ 1. – C. 38 — 40.
  5. Lakyh G.F. Byometpyia/G.F. Lakyh. – M.: Bblcsh. shk., 1990. – 352 c.
  6. Mahyatyc T. Molekyliaphoe klohypobahye/T. Mahyatyc., E. Fpych, D. Cembpyk; nep. c ahrl. nod ped. A.A. Baeba. – M.: Myp, 1984. – 479 c.
  7. Metodychhi pekomehdatsii z mopfo-rehetychhoi idehtyfikatsii ykpaihcbkyx bdzhil/O.I. Metlytsbka, B.P. Polischyk, I.I. Golobetsbkyj, M.D. Palbkiha. – Poltaba: Actpaia, 2014. – 30 c.
  8. Ploxyhckyj H.A. Byometpyia/H.A. Ploxyhckyj. – M.: Koloc, 1969. – 368 c.
  9. Shebahiha O.B. Metody henapametpychhoi ctatyctyky. Ppaktykym z biometpii/O.B. Shebahiha, C.C. Kpamapehko, B.M. Gahrahob. – Mykolaib:MDAY, 2008. – 165 c.
  10. Genomic correlates of recombination rate and its variability across eight recombination maps in the western honey bee (Apis mellifera L.)/C.R. Ross, D.S. DeFelice, G.J. Hunt et al.//BMC Genomics. – 2015.– V. 107, № 16. – P. 1 — 11.
  11. Magnus R.M. Mitochondrial DNA diversity of Honey Bees (Apis mellifera) from unmanaged colonies and Swarms in the Unated States/R.M. Magnus, D.T. Amber, A.L. Szalansky//Biochem. Genetic. – 2014. – V. 52. – P. 245 — 257.
  12. MEGA-4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0/K. Tamura, J. Dudley, M. Nei, S. Kumar//Molecular Biology and Evolution. – 2004. – V. 24. – P. 1596 — 1599.
  13. Peacall R. GENALEX-6: genetic analisys in Excel Population genetic software and research/R. Peacall, P.E. Smouse//Molecula Ecology Notes. – 2006. – № 6. – P. 288 — 295.
  14. Reaction of individual physiological barriers in bacterial infection in different races of the honeybee Apis mellifera/E.S. Saltykova, G.V. Ben’kovskaya, L.R. Gaifullina et al.//J. of Evolutionary Biochemistry and Physiology. – 2005. – V. 41, № 3. – P. 318 — 324.
  15. Rogstad S. GELSTATS: a computer program for population genetics analyses using VNTR multilocus probe data/S. Rogstad, S. Pelican//Bio Techniques. – 1996. – V. 21, № 6. – P. 187 — 196.
  16. Shaibi T. A microsatellite DNA toolkit for studying population structure in Apis mellifera/T. Shaibi,H. Lattorff, R. Moritz//Mol. Ecol. Resour. – 2008. – V. 8, № 5. – P. 1034 — 1036.
  17. Swofford D. BIOSYS-1: a Fortan programs for the comprehensive analysis of electroforetic data in population genetics and systematics/D. Swofford, R. Selander//J. Heredity. – 1981. – V. 72. – P. 281 — 283.