11. Apozygotism as a new method of enrichment of gene pool of sugar beet

Roik M. V., Koval'chuk N.S., Lysenko V.F., Himich N.M.
Pages: 49-52.

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Abstract
The purpose. The main objective of the study is enriching national gene pool sugar beet with entirely new source material with apozygotic way of seed reproduction based on a genetic model that provides differentiation of progeny, including according to apozygosis type. Methods. A method for sterile materials reproduction under non-pollen regime for obtaining new monogerm genotypes of sugar beet was used. Results. A genetic model differentiation of generative and somatic embryogenesis in apozygosis, which exploits selective genetic markers, is developed. Variability in apozygotic progeny is determined by phenotypic characteristics of nuclear genes of monogermity and cytoplasmic male sterility (CMS). Conclusions. We selected homozygous pollen-sterile lines of the first apomictic reproduction, which were further investigated on nuclear DNA quantitative content using "Partec" cytometry methods.

Key words: apozygosis; embryogenesis; sugar beet; monogermity; sterility; ploidy; genotype; phenotype.

References
  1. Balkob Y.Ja. Celektsyia caxaphoj cbeklbl ha retepozyc.//- M., Poccelbmash - 1978- 178 c.
  2. Boromolob M.A. Ycnolbzobahye anomyktychhblx MC lyhyj npy cozdahyy rybpydob caxaphoj cbeklbl./M.A. Boromolob//Caxaphaia cbekla. — 2012. — №9. — C. 27-30.
  3. Boromolob M.A. Ocobehhocty ycnolbzobahyia anomykcyca y caxaphoj cbeklbl npy cozdahyy hoboro ycxodhoro matepyala./M.A. Boromolob//Caxaphaia cbekla. — 2008. — №5. — C. 18-20.
  4. Zhyzhzhaloba T.P. Penpodyktybhaia byoloryia caxaphoj cbeklbl/T.P. Zhyzhzhaloba, B.B. Zhamehckaia, O.A. Podbyryha, G.Y. Japmoliuk. — Bopohezh., 2006. — C. 232.
  5. Zhyzhzhaloba T.P. Gehetycheckaia pazhokachectbehhoctb cemiah y metodbl ee npeodolehyia./T.P. Zhyzhzhaloba, O.A. Podbyryha//Caxaphaia cbekla. 2011. - №7. — C. 14-17.
  6. Maletskyj C.Y. Hacledobahye npyzhaka TsMC b anozyrotycheckyx notomctbax caxaphoj cbeklbl./C.Y. Maletskyj, E.Y. Maletskaia, C.C. Judahoba//Ehtsyklonedyia poda Beta. Byoloryia, rehetyka y celektsyia cbeklbl, YTsyG CO PAH, Pocyia; Yhctytyt caxaphoj cbeklbl, Ypayha. — Hobocybypck: “Coba”. — 2010. — C. 205-216.
  7. Maletskyj C.Y. Odhopoctkoboctb cbeklbl (embpyoloryia, rehetyka, celektsyia)./C.Y. Maletskyj, Ju.H. Shabpykob, C.G. Benpeb y dp. — Hobocybypck: Hayka. Cyb. otd-hye, 1988. — C. 168
  8. Poik M.B. Ahaliz mihlybocti pibhia nloidhocti rehomy byxidhyx celektsijhyx matepialib tsykpobyx bypiakib z bykopyctahhiam texholorij ahalizatopa nloidhocti “Partec”/M.B. Poik, H.C. Kobalbchyk, L.B. Alekcijchyk. — K., 2006. — C. 39.
  9. Jatseba O.A. Jakictb hacihhia tsykpobyx bypiakib, otpymahoro shliaxom anozyrotii./ O.A. Jatseba//Zb. hayk, npatsb ITsB YAAH. — K.: Arpapha hayka. — 2011. — C. 101-107.
  10. Owen F.V. Cytoplasmically inherited male sterility in sugar beet./F.V. Owen//Agric. Res. — 1945. — V. 71 (10). — P. 423-440.