14. Efficiency of use of optical and potentiometric immunobiosensors for determination of Salmonella typhimurium

https://doi.org/10.31073/agrovisnyk201603-14
Ogorodnijchuk Ju. O.
Pages: 69-71.

Full article: 
Abstract
The purpose. To analyze efficiency of the developed optical and potentiometric immunobiosensors on the basis of phenomena of surface plasmon resonance (SPR), ellipsometry of complete internal reflection (ECIR) and ionselective unipolar transistors (IsUT) for determination of Salmonella typhimurium in modelling solutions. Methods. Modified procedure on the basis of response of antigen-antibody, which includes processing of transdusers with protein A for creation of oriented stratum of antibodies. Results. Sensitivity of SPR-immunobiosensor made 101 – 107 kl/ml, ECIR-biosensor — less than 5 kl in 10 ml, IsUtbiosensor — from 2 to 5·105 kl/ml. Conclusions. Analyzed immunobiosensors are effective at determination of S. typhimurium in modelling solutions. The best sensitivity matching practical demands, was fixed for optical immunobiosensor on the basis of ECIR. IsUT-immunobiosensor has a lower sensitivity, but it differs by stability and high reproducibility of results. Immunobiosensors on the basis of SPR ensure sweeping analysis in real time and do not require special staff, therefore it is possible to use them for primary expressdiagnostics of S. typhimurium.

Key words: immunobiosensor, antigen-antibody response, Salmonella typhimurium.

References
  1. Biosensors for detection of pathogenic bacteria/D. Ivnitski, I. Abdel-Hamid, P. Atanasov, E. Wilkins//Biosensors & Bioelectronics. – 1999. V.14. – P. 599–624.
  2. Biosensors: A Novel Approach for Pathogen Detection/Syam R., Davis K.J., Pratheesh M.D. [et al.]//VETSCAN. – 2012. – Vol. 7 (1). – P. 14-18.
  3. Detection of Salmonella enteritidis using a miniature optical surface Plasmon resonance biosensor/Son J. R., Kim G., Kothapalli A. [et al.]//J. Physics. – 2007. – V. 61. – P. 1086-1090.
  4. Development of indirect competitive ELISA for the detection of Salmonella typhimurium/Bang J., Shukla S., Kim Y., Kim M.//Romanian Biotechnological Letters. – 2012. – V.17 (2). – P 7194-7204.
  5. Lazcka O. Pathogen detection: A perspective of traditional methods and biosensors/F. Javier Del Campo, F. Xavier Munoz.//Biosensors and Bioelectronics. – 2007. V 22. P. 1205–1217.
  6. Mandate of the Ministry of Health of Ukraine №400 12.05.2010. On approval of the State sanitary rules and regulations “Hygienic requirements to drinking water intended for human consumption” [Electronic source]:/Verkhovna Rada of Ukraine1994-2015. – Access mode: http://zakon4.rada.gov.ua/laws/show/z0452-10/page.
  7. Prusak-Sochaczewski E. An improved ELISA method for the detection of Salmonella typhimurium/Prusak-Sochaczewski E., Luong J.H.T.//J. Appl. Microbiology. – 1989. – V. 66 (I 2). – P. 127–135.
  8. Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium/Oh, B.K., Kim, Y.K., Park, K.W. [et al.]//Biosensors and Bioelectronics. – 2004. – V.19. – P. 1497–1504.
  9. The label free detection of aflatoxin using ellipsometry immunosensor/Nabok A., Mustafa M.K., Tsargorodskaya A., Starodub N.F.//Bioanalytical Chem. Manuscript ID: ac-2010-003739, 2010.
  10. Turner A. Biosensors: Fundamentals and Applications./Turner A, Karube I, Wilson G. – M.: Mir, 1992. – 614 p.
  11. Zaritskiy A. Salmonellosis/Zaritskiy A.– K.: Zdorovya. 1988. – 160p.