06. Genetic material of producers of cattle of Volynsk meat breed in bank of genetic resources of animals of M. Zubets Institute of animal husbandry and animal genetics of NAAS

https://doi.org/10.31073/agrovisnyk201607-06
Podoba B. Je., Dzhus P. P., Sydorenko O. V., Dobrians'ka M. L., Vyshnevs'kyj L. V., Kuzebna N. M., Janko T. S., Kopylova K. V.
Pages: 34-37.

Full article: 
Abstract
The purpose. Immunogenetic and molecular analysises of sperm of bulls of Volynsk meat breed which is stored in bank of genetic resources of animals of M. Zubets Institute of animal husbandry and animal genetics of NAAS. Methods. Determination of blood groups of producers by erythrocyte antigens of 9 systems (A, B, C, F, I, L, M, S, Z) and alleles of system B. Polymorphy of locuses CAPN 1530 and TG 5 of genes of kalpain and thyreoglobulin was determined using polymerase-chain reaction with the subsequent electrophoretic separation of restricting fragments of DNA in agarose gel. Results. Individual immunogenetic and molecular analysises were carried out of13 bulls of Volynsk meat breed which biological material was included for long-term storage. Rare for cattle carrying agents of antigen of M were fixed. Frequency of desirable allele G of gene of kalpain at probed animals made 0,654, of desirable allele T of gene of thyreoglobulin — 0,269. Conclusions. Obtained data will enable to forecast driving the genetic information in generations of offsets of probed producers.

Key words: Volynsk meat breed, genetic material, blood groups, genes of kalpain and thyreoglobulin.

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