Taran O. P., Lisovyj M. M., Bondus R. O.
Pages: 16-20.

Full article: 

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Abstract

The purpose. To determine biological characteristics of 2 isolates of Y-virus of potato, belonging to different groups of strains. One of isolates caused necrotic symptoms on plants of potato, another — of soft mosaic. Methods. Bioassays on plant-indicators. Direct methods of research of viruses: electronic microscopy withnegative contrasting, DAS-ELISA for research and identification of the revealed isolates. Results. There were revealed 2 isolates of Y-virus of potato, one of which caused striped mosaic, another — light mosaic. Necrotic isolate which caused light mosaic on host-plant — potato, caused symptoms of system necrosis on display plants of N. tabacum (cultivar Samsun). Usual isolate which induced light mosaic on plants of potato, on plant-indicators caused mosaic. Conclusions. Biological characteristics of 2 isolates of Y-virus of potato are investigated. It is determined that according to phenotypical features of infection of isolates on plant-indicators these isolates belong to different strain groups — necrotic and usual strains. The further molecular research of phylogenetic relationship of the revealed isolates is necessary.

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Key words: potato, tobacco, Y-virus of potato, necrotic strain, display plants, immunoenzymatic analysis.

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