11. Microclonal propagation black currant

https://doi.org/10.31073/agrovisnyk201612-11
Medvedieva T. V., Triapitsyna N. V., Natal'chuk T. A., Zapol's'kyj Ja. S.
Pages: 47-50.

Full article: 
Abstract
The purpose. To optimize separate stages of microclonal propagation black currant and to develop complex techniques of the sped up propagation for new cultivars on the basis of biotechnological methods. Methods. Preparation of nutrient mediums, and cultivation of plants in vitro was made under practical standards. Results. Specific features of deriving aseptic crop black currant of cultivar Saniuta and elite form 01 - 1 - 9 are probed. Nutrient mediums for proliferation and rootage in crop in vitro and methods of acclimatization to conditions ex vitro are selected. Positive influence of Acidum ascorbinicum upon efficiency of cultivation of microscions is shown. Conclusions. For deriving aseptic crop black currant as explants the most suitable are apical and lateral buds got from green scions. Addition of Acidum ascorbinicum (0,57 mM) into medium for initiation of aseptic crop and proliferation prevents depression of recovery and growth activity of explants which is caused by oxidation of phenols. Adaptation on substrate «Domoflor mix 3» ensures neogenesis 91 – 98% of microscions.

Key words: European black currant, aseptic crop, explant, in vitro.

References
  1. Silenko V.O. Suitability of currant varieties (Ribesnigrum L.) of NUBiP Ukraine selection for mechanized picking of berries/VA. Silenko, P.M. Hawaii//Graduate study and protection of rights to plant varieties. - 2013. - No. 3. - P. 13 - 17.
  2. Klyuddenko A.A. Features of obtaining aseptic culture Ribes nigrum L.A.A. Klyuddenko, S.Yu. Bilous, O.V. Overchko//Scientific. add NUBIP - 2014 - 9 p.
  3. Kolbanova E.V. Micropropagation method of black currant in vitro/E.V. Kolbanova, N.V. Kukharchik//Fruit growing: Sat. scientific Art./Institute of Fruit Production of the National Academy of Sciences of Belarus. - Samokhvalichy, 2006. - T. 2. - Ch. 2. - P. 163 - 168.
  4. In vitro propagation of black currant 'PerlaNeagra' and 'Amurg' cultivars/D. Clapa, A. Fira, C Plop, A. Bergs//Scientific papers of the RIFGPiteshti. - 2009. - V. XXV. - P. 207 - 212.
  5. Repeat D. Micropropagation as a means of rapid multiplication of newly-developed blackberry and black currant cultivars/D. Ruźić, T. Lazić//Agriculturae Conspectus Bibliography Scientificus. - 2006. - V. 71. - No. 4. - P. 149 - 153.
  6. Butenko R.G. The culture of isolated tissues and physiology of plant morphogenesis/R.G. Butenko - M., 1964. - 272 p.
  7. Skovorodnikov D.N. Clonal micropropagation in accelerating the selection process of currant black//Scientific paper. Vedic Series Natural Sciences. - 2012. - No. 21 (140). - P. 58 - 61.
  8. Clonal reproduction of plants of black currant (Ribesnigrum L.) in vitro/GK. Orazbaeva V.T. Khasanov, AR Iskakov, V.K. Shvidchenko//Vestn. science KazatU them. S. Seyfullin. - 2012. - No. 1 (72). - P. 245 - 257.
  9. Matushkina O.V. Features of currant multiplication in vitro/O.V. Matushkina, I.N. Pronina//Modern condition of the culture of currant and gooseberry: Sat. scientific tr - Michurinsk, 2007. - P. 285 - 289.
  10. Medvedeva T.V. Problems of acclimatization of cultured in vitro plants/T.V. Medvedev//Physiology and biochemistry of cultivated plants. - 2008. - Vol. 40, No. 4. - P. 299 - 309.