Ryzhenko G. F., Gorbatiuk O. I., Andriiaschuk V. O., Zhovnir O. M., Uhovs'ka T. M., Tiutiun S. M., Rieznichenko L. S., Dybkova S. M., Gruzina T. G.
Pages: 36-42.

Full article: 

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Abstract

The purpose. To determine perspectives and expediency of use of nano-particles of metals in biogeotechnology of manufacture of vaccines, to determine stimulating densities nano-particles of gold, silver, copper, and iron for vaccine strains of bacteria. Methods. Microscopic, bacteriological, biological, chemical, variation-statistical. Results. The expediency of application of colloidal solutions of nano-particles of metals (gold, silver, copper, and iron), synthesized by method of chemical condensation, as perspective for biogeotechnology of manufacture of vaccines is justified. Biosafety of the selected AuNP, AgNP, FeNP, and CuNP is confirmed by negative tests on cyto- and genotoxicity, and also mutagenicity. Their individual densities which make active metabolic processes in cells of vaccine strains of causal organisms and promote increase of volumes of bacterial mass at manufacture of vaccines are specified. Conclusions. It is expedient to use synthesized by method of chemical condensation colloidal solutions of AuNP, AgNP, FeNP, and CuNP in biogeotechnology of manufacture of vaccines as growth stimulants of cells of vaccine strains of microorganisms

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Key words: nano-particles of metals, biosafety, cytotoxicity, geno-toxicity, mutagenicity, H+-ATF activity, bacterial mass, vaccine.

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