Balashova G. S., Lavrynenko Ju. O., Kotov B. S.
Pages: 51-55.

Full article: 

---

Abstract

The purpose. To determine the optimum technological methods influencing heightening intensity of tuber-formation of potato of early ripening variety Kobza in crop of stolons in vitro. Methods. Complex use of laboratory, mathematical-statistical, calculation-comparative methods and system analysis. Results. Experimental data are resulted on influence of density of saccharose and aucsins in nutrient medium on induction of tuber-formation at reproduction of potato in crop of stolons in vitro. Conclusions. The maximum productivity and reward payment of holdings at determination of optimum elements of technique of growing of microtubers of early ripening variety of potato Kobza in crop of stolons in vitro is generated at adding in nutrient medium of saccharose in dose of 80 g/l and kinetin in dose of 0,5 mg/l. Mass of average microtuber is 53,2 mg, amount of stolons which have organized microtubers – 87% at the cost price of microtuber 2,49 hrn and profitableness of production 141%.

---

Key words: crop of stolons in vitro, tuber-formation, amount of stolons, height of plants, saccharose, kinetin, nutrient medium, mass of microtubers.

---

References

1. Vnedrenie sistemy semenovodstva kartofelja na ozdorovlennoj osnove v Respublike Tatarstan/F.F. Zamalieva, G. F. Safiullina, R. R. Nazmieva [i dr.]//Kartofelevodstvo v regionah Rossii : aktual'nye problemy nauki i praktiki/VNIIKH. — M., 2006. — S. 167-181.
2. Semenovodstvo — na ozdorovlennuju meristemnuju osnovu/R. G. Gareev, F. F. Zamalieva,A. S. Zajnulina [i dr.]//Kartofel' i ovoshhi. — 2001. — №1. — S. 9-10.
3. Optimizacija priemov ozdorovlenija, razmnozhenija i zashhity semennogo kartofelja ot virusnoj infekcii : metod. ukazanija/BelNIIZR. — Minsk, 1996. — 16 s.Almasi A. Photosintetic aleration of virus infected plants/A. Almasi, A. Harsanyi, R. Gaborjanyi//Acta Phytopathol Acad. — 2001. — Vol. 36. — N½. — P. 15-29.
4. Vozmozhnosti ispol'zovanija immunohromatograficheskih test-sistem dlja diagnostiki virusov kartofelja / D. V. Kravchenko, A. I. Uskov, Ju. A. Varicev [i dr.]//Kartofelevodstvo : sb. nauch. tr. : materialy koordinac. soveshh. i nauch.-prakt. konf., posvjashh. 120-letiju so dnja rozhdenija A. G. Lorha/RASHN, VNIIKH ; pod red. E. A. Simakova. — M., 2009. — S. 208-213.
5. Sovershenstvovanie virusologicheskogo kontrolja v processe formirovanija i podderzhanija banka zdorovyh sortov kartofelja/B. V. Anisimov, E. V. Ovjes, O. V. Topisheva [i dr.]//Kartofelevodstvo : sb. nauch. tr. : materialy koordinac. soveshh. i nauch.-prakt. konf., posvjashh. 120-letiju so dnja rozhdenijaA. G. Lorha/RASHN, VNIIKH ; pod red. E. A. Simakova. — M., 2009. — S. 188-192.
6. Sologub A.S. Sposob biohimicheskoj identifikacii sortov kartofelja/A. S. Sologub, P. A. Mel'nik//Kartofelevodstvo : sb. nauch. tr. : materialy koordinac. soveshh. i nauch.-prakt. konf., posvjashh. 120-letiju so dnja rozhdenija A. G. Lorha/RASHN, VNIIKH ; pod red. E. A. Simakova. — M., 2009. — S. 178-182.
7. Agrobakterial'naja transformacija sortov kartofelja ukrainskoj selekcii SRY-genami, obespechi- vajushhimi ustojchivost' k nasekomym- vrediteljam/V. N. Zhuk, T. N. Olijnyk, A. I. Emec [i dr.]//Kartofelevodstvo : sb. nauch. tr. — Minsk, 2008. — T. 14. — S. 67-73.
8. Hal R. Biotehnologija s.-h. rastenij/R. Hal ; per. s angl. — M. : Agropromizdat, 1998. — 498 s.
9. Awan A.R. In vitro elimination of potato leaf roll polerovirus from potato varieties/A. R. Awan, S. M. Mughal//European Journal of Scientific Research. — 2007. — Vol. 18. — N1. — P. 155-164.
10. Differential organ infection studies, potyvirus elimination and field performance of virus-free garlic plants produce by tissue culture/R. Ramirez-Malagon, L. Perez-Moreno, A. Borodanenko [and use]//Plant Cell Tiss Organ Cult. — 2006. — Vol. 86. — P. 103-110.
11. Skoog F. Chemical regulation of growth and organ formation in plant tissues cultured in vitro/F. Skoog, C. O. Miler//In The Biological Action of Growth Substances : symposia of Societi for Experimental Biologi. — Cambridge : Cambridge University Press, 1957. — N. 11. — P. 118-153.
12. Biotehnologicheskie metody poluchenija i ocenki ozdorovlennogo kartofelja : metodicheskie rekomendacii ; podgot. : L. N. Trofimec, V. B. Bojko, T. V. Zejruk [i dr.]. — M., 1988. — 37 s. s.
13. MetodichnI rekomendatsiyi schodo provedennya doslidzhen z kartopleyu ; pIdgot. : V. S. Kutsenko, A. A. Osipchuk, A. A. Podgaetskiy [ta in.]/in-t kartoplyarstva. — Nemishaeve, 2002. — 183 s.
14. Ozdorovlennya kartopli v kulturi in vitro: naukovo-metodichni rekomendatsiyi; pIdgot.: R. A. Vozhegova, Yu. O. Lavrinenko, G. S Balashova [ta in.]/in-t zrosh. zemlerob. — Herson, 2013. — 20 s.
15. Optimizacija priemov ozdorovlenija, razmnozhenija i zashhity semennogo kartofelja ot virusnoj infekcii: metod. ukazanija / BelNIIZR. — Minsk, 1996. — 16 s.
16. Biotehnologicheskie metody poluchenija i ocenki ozdorovlennogo kartofelja : metodicheskie rekomendacii; podgot.: L. N. Trofimec, V. B. Bojko, T. V. Zejruk [i dr.]. — M., 1988. — 37 s.
17. Metodika polovih i laboratornih doslidzhen na zroshuvanih zemlyah/[R. A. Vozhegova, Yu. O. Lavrinenko, M. P. Malyarchuk ta in.] ; za red. R. A. Vozhegovoyi/in-t zrosh. zemlerob. — Herson, 2014. — 286 s.