10. Toxicological biotesting of waters of surface sources of water service and drinking water using larvas of Хenopus laevis

https://doi.org/10.31073/agrovisnyk201802-10
Arystarhova E. O.
Pages: 60-63.

Full article: 
Abstract
The purpose. Heightening selfdescriptiveness of the method of biotesting of waters of surface sources of water service and drinking water due to use of larvas of Xenopus laevis. Methods. Testing on response of fixation of 3-week larvas (tadpoles) of Rana ridibunda Pallas and Xenopus laevis Daudin with 8-day exposure of larvas. Results. During biotesting the low level of chronic toxicity of waters under test was fixed on tadpoles of R. ridibunda (25 – 50%), low level of acute lethal toxicity (25 – 50%) and average level of chronic toxicity (50 – 75%) — on tadpoles X. laevis. Conclusions. The probes confirm an opportunity of heightening selfdescriptiveness of the method of biotesting of waters of sources of water service and drinking water with the use of larvas of Xenopus laevis, which have appeared in conditions CE «Zhytomyrvodokanal» more sensitive to rather low levels of pollution (on the average for 28,60% in acute experience at duration of 4 day, and for 28,86% in short-term chronic experience at duration of 8 day) in comparison with larvas of Rana ridibunda.

Key words: waters of sources of water service, drinking water, biotesting, larvas of R. ridibunda and X. laevis, fixation, toxicity level.

References
  1. Osmalenyi M.S., Holovkov A.M., Naniieva A.V., Verholias M.R. (2015). Kompleksna otsinka toksychnosti vodnykh zrazkiv za dopomohoiu roslynnykh i tvarynnykh test-orhanizmiv. Faktory eksperymentalnoi evoliutsii orhanizmiv. T.16. S. 74-77. [In Ukrainian].
  2. Honcharuk V.V. Patent 10804 A Ukraina, MPK G 01 N 33/18; G 01 N 21/76. Sposib kompleksnoho vyznachennia henetychnoi bezpechnosti pytnoi vody ; zaiavnyk ta patentovlasnyk V.V. Honcharuk; zaiavl. 5.11.2015, opubl. 11.04.2016, Biul. № 7. [In Ukrainian].
  3. Szczerbinska N., Galczynska M. (2015). Biological methods used to assess surface water quality. Arch. Pol. Fish. № 43. P. 185-196.
  4. Radic S., Gregorovic G., Stipanicev D., Cvjetko P. et al. (2013). Assessment of surface water in the vicinity of fertilizer factory using fish and plants. Ecotoxicology and Environmental Safety. № 96. P. 32-40.
  5. Skok S.V. (2015). Otsiniuvannia yakosti pytnoi vody m. Khersona metodom biotestuvannia. Ahroekolohichnyi zhurnal. № 2. S.26-30. [In Ukrainian].
  6. Stetsiuk L.M. (2013). Vykorystannia metodiv bioindykatsii ta biotestuvannia dlia otsinky stanu vodnykh ekosystem. Visnyk Natsionalnoho universytetu vodnoho hospodarstva ta pryrodokorystuvannia. Vyp. 2 (62). «Silskohospodarski nauky». S.175-181. [In Ukrainian].
  7. ISO 10706: 2000 Water quality. Determination of long term toxicity of substances to Daphnia magna Straus (Cladocera, Crustacea). – 26 p.
  8. Arystarkhova E.O. (2017). Ekspres-otsinka potentsiinoi nebezpeky vody metodom biotestuvannia na Daphnia magna S. Visnyk ahrarnoi nauky. № 2. S. 50-54. [In Ukrainian].
  9. Arystarkhova E.O. (2017). Ekspres-otsinka toksychnosti vod poverkhnevykh dzherel vodopostachannia z vykorystanniam ryb Danio rerio. Rybohospodarska nauka Ukrainy. № 3 (41). S. 17-25. [In Ukrainian].
  10. DSanPiN 2.2.4-171-10 «Hihiienichni vymohy do vody pytnoi, pryznachenoi dlia spozhyvannia liudynoiu» / № 452 / 17747. – MOZ Ukrainy. Derzhavni standartni normy ta pravyla / [chynnyi vid 1.07.2010 r.]. – 50 s. [In Ukrainian].