Hromyh N. O., Matiuha V. L., Lyholat Ju. V., Lisovyj M. M., Nazarenko M. M., Grygoriuk I. P.
Pages: 20-25.

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Abstract

The purpose. To study properties of grain of hybrid of corn Orzhitsa 237 MV and action of ferments antioxidant protection for detection of aftereffects of treatment of sowings with selective herbicides Kharnes, Proponit, Master and Stellar. Methods. Activity of superoxide scavenger (SOD; KF 1.15.1.1) was determined according to the level of retardation of regenerative process of nitrosine tetrazolium at presence of nicotinamidine nucleotide and phenazine methasulphate. Catalase activity was determined by titrometric method with solution of potassium permanganate after incubation of supernatant during 30 minutes at 25°C with hydrogen superoxide and it was expressed in mM of H2O2/min g of crude mass. Content of GSH and activity of glutathione-S-transferase was determined according to practical standard. Results. After-effect of herbicidal treatment on the dead-ripe grain of hybrid of corn Orzhitsa 237 MV is probed. Lower mass of seeds and content of water-soluble proteins depending on individual properties of herbicides is fixed. There were also determined different direction changes in activity of anti-oxidant ferments. Conclusions. The determined morphological and metabolic changes can be evaluated as development of after-effect of herbicides: aberration from control indexes of seeds, caused by action of herbicides, points out to aggravation of yield of probed hybrid. Results of investigation specify expediency of testing perspective hybrids also on responsivity to herbicides with different mechanisms of action.

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Key words: hybrid of corn, after-effect of herbi­cides, anti-oxidant ferments, rebuilt glutathione, activity of glutathione-S-transferase.

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