04. Improvement of diagnostic tools and specific prophylaxis of Teshen disease

https://doi.org/10.31073/agrovisnyk201804-04
Derev’ianko S. V., Reshot'ko L. M., Bova T. O., Dmytruk O. O., Golovko A. M., Katsymon V. V.
Pages: 26-30.

Full article: 
Abstract
The purpose. To update diagnostic means and methods of specific prophylaxis of Teshen disease according to recommendations of International epizootic bureau. Methods. Reference and production strains of Tesho-virus of pigs are used. Accumulation of viruses was carried out in re-vaccinating cultures of kidney cells of an embryo of pig and newborn Syrian hamster. In experiments they used virus neutralization reaction, immunofluorescence, immunoassay, enzyme-linked immunosorbent assay, polymerase chain reaction with reverse transcription. Results. Sets of diagnosticums of Teshen diseases and inactivated vaccine against it with the characteristics which considerably exceeding domestic and foreign analogues are created on the basis of new domestic strain Teschovirus A «Dneprovskii-34». Conclusions. Diagnostic means and methods of specific prophylaxis of Teshen disease are updated.

Key words: Teshen disease, polymerase chain reaction, immunofluorescence analysis, immunoproxidase method, immunofluorescence analysis, virus, vaccine.

References
  1. Virus Taxonomy: The Classification and Nomenclature of Viruses The Online (10th) Report of the ICTV. (2017). URL: https://talk.ictvonline.org/ictv-reports/ictv_online_report/
  2. Romanenko V.F., Soroka V.Y., Pruss O.H. (1992). Rekomendatsii po diahnostike i meram bor'by s enzootychesksm tntsefalomyelstom (bolezn'yu Teshena) sviney. Kiev – 17 p.
  3. Witte von K.H. Auerbach J., Loss K.U., Neuhaus S. & Prager D. (1994). Typisierung von 17 porzinen Enterovirusisolaten aus Polioenzephalomyelitisfällen der Jahre 1983–1991. DTW Dtsch. Tierarztl. Wochenschr. Vol. 101. P. 453–492.
  4. OIE. (2008). Manual of Standarts for Diagnostic Test and Vaccinees. 6 Ed. Paris. 957 p.
  5. Buzun A.Y., Babkin M.V. (2000). Podtipovye razlichiya sredi shtamov i yzolyatov vozbuditelya bolezni Teshena. Veterinarna meditsina. T. 78. P. 23–28.
  6. Holovko A.M., Derevianko S.V., Bova T.O., Soroka V.I., Katsymon V.V. (2009). Konstruyuvannya vydospetsyfichnykh praymeriv dlya molekulyarno-henetychnoyi identyfikatsiyi teshovirusiv ta enterovirusiv A i V. Mizhvidomchyy tematychnyy nauk. zb.: Sil's'kohospodars'ka mikrobiolohiya. Chernihiv: TsNTEI. Vyp. 10. P. 156-165.
  7. Holovko A.M., Katsymon V.V., Derevianko S.V. et al. (2011). Molekuliarno-henetychna identyfikatsiia teshovirusiv ta enterovirusiv svynei A i V. Visn.ahrar. nauky..№ 11. C. 33 – 37.
  8. Volkova I.V., Bova T.O., Derevianko S.V. Pat. 58734 Ukrayina, MPK G01N 33/53 (2006.01) Sposib oderzhannya hiperimunnoyi syrovatky krovi do virusiv tvaryn i roslyn. Zayavnyk i patentovlasnyk Instytut sil's'kohospodars'koyi mikrobiolohiyi NAAN. № u201011151; zayavl. 17.09.2010; opubl. 26.04.2011. Byul. № 8.
  9. Bova T.O., Soroka V.I., Derevianko S.V., Reshot'ko L.M., Bozhok L.V., Holovko A.M., Katsymon V.V. (2014). Metodychni rekomendatsiyi z virusolohichnoho monitorynhu enzootychnoho entsefalomiyelitu (hvoroby Teshena) svyney. Instytut sil's'kohospodars'koyi mikrobiolohiyi ta ahropromyslovoho vyrobnytstva NAAN, Chernihiv: ChDTsNII. 19 s.
  10. Derevianko S.V., Soroka V.I., Bokun A.O., Bozhok L.V., Bova T.O. Pat. 57372 Ukrayina, MPK (2011.01) S12N 7/00, A61K 39/125, A61K 39/187 (2011.01), C12R 1/92 (2006.01). Shtam Porcine teschovirus dlya vyrobnytstva veterynarnykh imunobiolohichnykh preparativ. Zayavnyk i patentovlasnyk Instytut sil's'kohospodars'koyi mikrobiolohiyi NAAN. № u201009325; zayavl. 26.07.2010; opubl. 25.02.2011, byul. № 4.