Zapol's'kyj Ja. S., Medvedieva T. V., Natal'chuk T. A., Bublyk M. O.
Pages: 45-50.

Full article: 

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Abstract

The purpose. To investigate influence of preparation Lizoformin 3000 against pathogenic microflora at obtaining aseptic cultures of honeysuckle (Lonicera edulis Turcz) and on the further regeneration of plants depending on method of sterilization and state of initial plants. Methods. Laboratory, mathematical, calculation-comparative. Results. Influence of sterilizing substances on obtaining aseptic cultures of honeysuckle in conditions in vitro is studied. Experiment were spent on grades: Alisia, Spokusa, Chaika, Nimfa, Dochka Veletnia, Karina. As sterilizing agents they used Lizoformin 3000 at different time of sterilization and solution of mercury bichloride as the control. Stages of sterilization on the control included the following components: 1) treatment of explants in solution of sodium hipochlorite — 20 min with subsequent washing in water; 2) sterilization with spirit (C2H5OH) — 20 sec with washing in water; 3) sterilization in solution of mercury bichloride (HgCl2) — 2 min with 3-times washing in sterile distilled water. In the variant with studying preparation Lizoformin 3000 instead of solution of mercury bichloride they used the studied preparation at expositions of 5, 7 and 10 min. Conclusions. For the maximal obtaining of sterile and viable explants the sterilizing agent and its toxicity has great value. Preparation Lizoformin 3000 at corresponding concentration and duration of sterilization should be recommended for obtaining aseptic cultures of honeysuckle. On the background of application of that preparation with exposition of 5 min they allocated by efficiency of regeneration 3 groups of varieties of honeysuckle: with high reclaiming ability (94 – 96%) — Alisia, Karina and Spokusa; with average reclaiming ability (86 – 87%) — Chaika and Dochka Veletnia, and with low reclaiming ability (80%) — Nimfa. Preparation Lizoformin 3000 in concentration of 3% and with duration of exposition of 5 min provides optimum efficiency of sterilization and regeneration of explants of honeysuckles and does not reduce their factors of duplication. Preparation Lizoformin 3000 at corresponding concentration and duration of sterilization should be recommended for obtaining aseptic cultures of varieties of honeysuckle.

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Key words: honeysuckle, sterilization, Lizo­formin, explant, in vitro, introduction in culture, proliferation.

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References

1. Akimova S.V., Semenova N.A., Vikulina A.N. (2013). Primenenie jetioljacii na razlichnyh jetapah mikroklonal'nogo razmnozhenija zhimolosti (Lonicera L.) podsekcii Saerulea Rehd. [Application of etiolation at various stages of microclonal reproduction of honeysuckle (Lonicera L.) subsection Saerulea Rehd.]. Trudy BGU. No. 8. P. 33-37. [in Russian].
2. Dziedzic E. (2008). Propagation of blue honeysuckle (Lonicera caerulea var. kamtscatica Pojark.) in in vitro culture. Journal of fruit and ornamental plant research. No. 16. P. 93–100.
3. Sedlák J., Paprštein F. (2007). In vitro propagation of blue honeysuckle. HORT. SCI. (PRAGUE). No. 34. P. 129–131.
4. Krupa-Małkiewicz M., Ochmian I. (2014). Propagation of blue honeysuckle (Loniceracaerulea L.) in in vitro culture. Journal of basic&applied sciences. No.10. P. 164–169.
5. Bljudneva E.A., Krickaja T.A., Kashin A.S. (2013). Ispol'zovanie klonal'nogo mikrorazmnozhenija dlja massovogo poluchenija posadochnogo materiala dekorativnyh i plodovo-jagodnyh kul'tur v Botanicheskom sadu SGU [The use of clonal micropropagation for the mass production of planting material for ornamental and fruit crops in the Botanical Garden of the Saratov State University]. Bul. of the Botanical Garden of the Saratov State University. Issue 11. P. 119-131. [in Russian].
6. Muratova S.A., Jankovskaja M.B., Shornikov D.G. (2005). Osobennosti vvedenija v kul'turu in vitro plodovyh i jagodnyh rastenij. [Peculiarities of the introduction of fruit and small fruit plants into the in vitro culture]. Plodovodstvo. Institute for Fruit Growing of the National Academy of Sciences of Belarus. Vol.17. Part 2. 182-185. [in Russian].
7. Vysockij V.A., Valikov V.A. (2014). Klonal'noe mikrorazmnozhenie zhimolosti v proizvodstvennyh uslovijah. [Honeysuckle clonal micropropagation under the manufacturing conditions]. Sadovodstvo i vinogradarstvo. 6. 18-19. [in Russian].
8. Hui JX, Wen SCh, Hua ZY, Ming LX. (2012). Comparative study on different methods for Lonicera japonicaThunb. micropropagation and acclimatization. J Med Plant Res. 6. P. 4389-4393.
9. Charkova A.A. (2013). Redkie i ischezajushhie vidy rastenij v kul'ture in vitro. Charkova A.A. Riedkiie i ischiezaiuschiie vidy rastienii v culture in vitro. [Rare and disappearing plants species in the in vitro culture]. Ogarev-online. No. 11. [in Russian].
10. Murashige T, Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15. P. 473-497.