10. Influence of melasses non-sugars on efficiency of fermentation of mash from sacchariferous raw material

https://doi.org/10.31073/agrovisnyk201903-10
Koval' O. O., Olijnichuk S. T.
Pages: 63-68.

Full article: 
Abstract
The purpose. To establish influence of melasses non-sugars on physiological activity of yeast and efficiency of fermentation of mash from sacchariferous raw material. Methods. Determination of physical and chemical structure of sacchariferous raw material, parameters of mash and mature distillers’ beer spent according to techniques which are used in production of sugar and spirit. Statistical. Results. At decrease of melasses share in nutrient medium the general high quality of a mash raises, concentration of dry substances in it decreases, reducing thus osmotic stress of cells of yeast, and, accordingly, charges of sugars on glycerin production. Reduction of non-sugars in a mash at replacement of melasses sugars with sugars of diffusion juice promotes on 25 and 50% greater accumulation of spirit in distillers’ beer, increases biomass in it and decreases parameter of non-fermented sugar. Increase in a share of sugars of diffusion juice in a mash up to 75% actually levels negative influence of melasses non-sugars on ability to live of yeast cell. Replacement of a part of melasses sugars with sugars of juice increases output of ethanol that enables in addition to receive 1,24–2,29 dal on each ton of raw material of this quality. Conclusions. It is established that melasses non-sugars negatively influence growth of yeast biomass and its alcohol-forming ability. Decrease in non-sugars in a mash because of replacement of melasses sugars with sugars of diffusion juice improves parameters of quality of mature distillers’ beer. Normative output of spirit from saccharose is reached at fermentation of diffusion juice with high quality of 84,9%. The highest effect of processing melasses of this quality can be reached by its mixing with diffusion juice in a proportion 25:75.


Key words: non-sugars, diffusion juice, fermentation, melasses, Saccharomyces cerevisiae, bioethanol, yeast.



References
  1. Zubchenko V., Hrushytskyi M., Tkachenko L. (2008). Vykorystannia napivproduktiv tsukrovoho vyrobnytstva yak alternatyvnoi syrovyny dlia oderzhannia bioetanolu Use of semiproducts of sugar production as an alternative raw material for bioethanol production. Kharchova promyslovist. Food Industry. 7. P. 12–14. in Ukrainian.
  2. Kaletnik H. (2010). Biopalyvo: prodovolcha, enerhetychna ta ekolohichna bezpeka ukrainy: monohrafiia. Biofuels: food, energy and ecological safety of Ukraine: monograph. Kyiv: Khai-Tek Pres. 516 p. in Ukrainian.
  3. Ivanov S. V., Sychevskyi M. P., Oliynichuk S.T., Yarchuk M. M., Kalinichenko M. F., Sosnytskyi V. V., Lukashevych Ye. A., Khomichak L. M., Shyian P. L., Rudakov V.K. Patent. 86054 Ukraina, MPK (2013.01) C10L 1/00, S13V 99/00. Sposib vyrobnytstva tsukru ta bioetanolu. Method of producing sugar and bioethanol; Applicant and Patent Attorney National University of Food Technologies, Research and Production Limited Liability Company Intermash, Institute of Food Resources of the National Academy of Agrarian Sciences of Ukraine. u20130777; stated. 19.06.13; Bjul. 10.12.13. 2 p. in Ukrainian.
  4. Ramesh Duraisam, Ketemaw Salelgn, Abiyu Kerebo Berekete. (2017). Production of beet sugar and bio-ethanol from sugar beet and it bagasse: a review. International Journal of Engineering Trends and Technology. Vol. 43. 4. P. 222–233. doi.org/10.14445/22315381/IJETT-V43P237
  5. Bowen E., Kennedy S. C., Clark W. M. (2010). Ethanol from sugar beets : a process and economic analysis. Worchester Polytechnic Institute. 143p. https://web.wpi.edu/Pubs/E-project/Available/E-project-042810-165653/unrestricted/Ethanol_from_Sugar_Beets_-_A_Process_and_Economic_Analysis.pdf
  6. Schieb, P.-A., Lescieux-Katir, H., Thénot, M., Clément-Larosière, B. (2015). Biorefinery 2030. Biorefinery 2030: Future Prospects for the Bioeconomy. Berlin, Heidelberg: Springer Berlin Heidelberg. 123p. doi.org/10.1007/978-3-662-47374-0
  7. Oliynichuk S. T., Tkachenko A. F., Shevchenko V. I., Koval K.O., Rudnichenko L. V., Shustikova N. Ye. Patent 20915 Ukraina, MPK S12N, C12P, C12R 1/865 Hibrydnyi shtam drizhdzhiv saccharomyces cerevisiae meyen dlia mikrobiolohichnoho syntezu spyrtu z meliasy. Hybrid strain of yeast Saccharomyces cerevisiae Meyen for microbiological synthesis of alcohol from molasses; Applicant and Patent Attorney Ukrainian Research Institute of Alcohol and Biotechnology of Food Products. 96124751; stated. 20.12.96; Bjul. 15.12.00. 2 p. in Ukrainian.
  8. DSTU 3696-98 (HOST 30561-98). (1998). Meliasa buriakova. Tekhnichni umovy. Beet molasses. Specifications Kyiv. 10 p. in Ukrainian.
  9. TTR 000 32744-3508-2005. (2005). Typovyi tekhnolohichnyi rehlament oderzhannia meliasno-spyrtovoi brazhky i presovanykh khlibopekarskykh drizhdzhiv. Typical technological regulations for the production of molasses-alcohol fermented wort and pressed baking yeasts. Kyiv. 246 p. in Ukrainian.
  10. Buhaenko Y.F. (1989). Tekhnokhymycheskyi kontrol sakharnoho proyzvodstva. Technochemical control of sugar production. Moskva: Ahropromyzdat, 1989. 216p. in Russian.
  11. Rukhliadieva A.P. (1974). Tekhnokhymycheskyi kontrol spyrtovoho proyzvodstva. Technochemical control of alcohol production. Moskva: Pyshchevaia promishlennost, 1974. 208 p. in Russian.
  12. Boiko L.M. (1986). Fyzyko-khymycheskye metodi kontrolia brodylnikh proyzvodstv. Physicochemical methods of controlling fermentation productions. Kyev: Tekhnyka. 192 p. in Russian.
  13. Kupchyk M. P., Reva L. P., Shtanheieva N. I., Cherniavska L.I., Lipiets A.A., Khomichak L.M. .... Nychyk O.V. (2007). Tekhnolohiia tsukrystykh rechovyn. Laboratornyi praktykum. Technology of sugar-containing substances. Laboratory Workshop. Kyiv: NUKhT. 393 p. in Ukrainian.
  14. Marynchenko V. O. Domaretskyi V.A., Shyian P.L., Shvets V.M., Tsyhankov P.S., Zholner I.D. (Ed. Marynchenko V. O.). (2003). Tekhnolohiia spyrtu: pidruchnyk. Alcohol technology: textbook. Vinnytsia : Podillia-2000, 2003. 496 p. in Ukrainian.
  15. Koval O. O., Oliynichuk S.T., Khomichak L.M., Lysak T.I., Batoh Yu.O. (2014). Zbrodzhuvannia tsukrovmisnykh produktiv tsukrovoho vyrobnytstva v bioetanol. Fermentation of sugar-containing products of sugar production into bioethanol. Tsukor Ukrainy. Sugar of Ukraine. 2 (98). P. 10–13. in Ukrainian.
  16. Domaretskyi V. A., Prybylskyi V. L., Mykhailov M. H. (Ad. Domaretskyi V.A.). (2005). Tekhnolohiia ekstraktiv, kontsentrativ i napoiv iz roslynnoi syrovyny: pidruchnyk. Technology of extracts, concentrates and beverages from plant raw materials: textbook. Vinnytsia: Nova knyha, 408 p. in Ukrainian.
  17. Nevoigt E., Stahl, U. (1997). Osmoregulation and glycerol metabolism in the yeast saccharomyces cerevisiae. FEMS Microbiology Reviews. Vol. 21. Issue 3. P.231–241. doi.org/10.1111/j.1574-6976.1997.tb00352.x