04. Detection of virus, bacterial and phytoplasmic diseases on vineyards of Odesa oblast

https://doi.org/10.31073/agrovisnyk201904-04
Konup A. I., Muliukina N. A., Konup L. O.
Pages: 24-29.

Full article: 
Abstract
The purpose. To determine presence of virus, bacterial and phytoplasmic diseases of grape on grape plantings of Odesa oblast. Methods. Field, laboratory. Fytosanitory diagnostic study of vineyards in grape-cultivation farms (Ovidiopol and Tatarbunar regions of Odesa oblast). They identified causal organisms of diseases of grape plants using serological (immunoenzymatic — ELISA) and molecular-biological (polymerase chain reaction (PCR) with electrophoretic detection) methods. Results. At visual diagnostic study of grape plantings in Ovidiopol region they detected plants with symptoms of leaf curl and on occasion — with symptoms similar to that caused by fanleaf virus and virus of a complex striation of wood of a grape. Grape plants with symptoms of bacterial cancer and phytoplasmic diseases are detected on vineyards of Ovidiopol and Tatarbunar regions. Presence of causal organisms of virus (fanleaf of grapevine, leaf curls, and viruses of a complex striation of wood of a grape), phytoplasmic diseases and bacterial cancer is confirmed by methods of ELISA and PCR. High scale of defeat is registered by the causal organism of bacterial cancer on scrubs of variety Cabernet Sovinion, by the causal organism of phytoplasmic disease of wood blackening — on variety Shardone. Conclusions. The greatest percent of plants of grape struck by virus and phytoplasmic diseases, has appeared in Ovidiopol region, by the causal organism of bacterial cancer — in Ovidiopol and Tatarbunar regions of Odesa oblast. It is established that approximately 10% of the determined diseases of grape are virus ones, nearby 25 are bacterial cancer, and 65% are phytoplasmic diseases.

Key words: viruses of grape, wood blackening, IFA, PCR, bacterial cancer of grape, grape.

References
  1. Almeida R.P.P., Daane K., Bell V. (2013). Ecology and management of grapevine leafrolldisease. Frontiers in Microbiology. V. 4, P. 94. doi:10.3389/fmicb.2013.00094
  2. Keldysh M. A. (2003).Virusy, viroidy i mikoplazmy rasteniy. [Viruses, viroids and plant mycoplasmas]. Moscow: PFUR, 157 p. [in Russian].
  3. Lemanova N.B., Gatina E.Sh. (1991). Bakterial'nyye bolezni vinograda i plodovykh kul'tur. [Bacterial diseases of grapes and fruit crops]. Chisinau: Shtiintsa, 116 p. [in Russian].
  4. Martelli G. P. (2012). Grapevine Virology Highlights: 2010–2012: Proceedings of the 17th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG). P. 13–31. October 7-14, 2012, Davis, California, USA. [in English].
  5. Vizitiu (Bălăşoiu) Diana, Dejeu L. (2011).Crown gall (Agrobacterium spp.) and grapevine. JOURNAL of Horticulture, Forestry and Biotechnology. V. 15(1), P. 130- 138.
  6. Borhsenius S.N., Chernova O.A. (1989). Mikoplazmy: molekulyarnaya i kletochnaya biologiya, patogennost', diagnostika. [Mycoplasmas: molecular and cellular biology, pathogenicity, diagnosis]. L.: Science, 156 p. [in Russian].
  7. Molecular biomarker analysis – General definitions and requirements for microarray detection of specific nucleic acid sequences European and Mediterranean Plant Protection Organization (EPPO). (2013). ISO 16578. http://www.eppo.org [in English].
  8. Esteban A., Engel E.A., Escobar P.F. (2009). A diagnostic oligonucleotide microarray for simultaneous detection of grapevine viruses. Journal of Virological Methods. V. 2, Is. 163. P. 445–451. doi: 10.1016/j.jviromet.2009.11.009
  9. Lee I.-M., Gundersen D.E., Hammond R.W., and Davis R.E. (1994). Use of Mycoplasmalike Organism (MLO) Group-Specific Oligonucleotide Primers for Nested-PCR Assays to Detect Mixed-MLO Infections in a Single Host Plant. The American Phytopathological Society. V. 84, P. 559-566. doi: 10.1094/Phyto-84-559
  10. Sahar A.Youssef, Yasmin Sayed, Osama S. Hassan, Gehan Safwat and A. A. Shalaby. (2017). Universal and Specific 16S-23Sr RNA PCR Primers for Identification of Phytoplasma associated with sesame in Egypt. International Journal of Advanced Research in Biological Sciences. V. 4, Is. 7. P. 191-200. doi: 10.22192/ijarbs
  11. Avramov Z., Gillet J., Laginova M. (2008). First Detection of Stolbur Phytoplasma in Grapevines (Vitis vinifera cv. Merlot) Affected with Grapevine Yellows in Bulgaria. J. Phytopathology. V. 156, P. 112 – 114. doi: 10.1111/j.1439-0434.2007.01339.x
  12. Haas Jerry H., Moore Larry W., Ream Walt, Manulis Shulamit. (1995). Universal PCR Primers for Detection of Phytopathogenic Agrobacterium Strains. Applied and Environmental Microbiology. V. 61. № 8. P. 2879 – 2884.
  13. Konup L.A., Gayday A.E. , Milkus B.N. (2001). Bakterial’nyy rak vinograda v Ukraine. [Bacterial cancer of grapes in Ukraine]. Visnik ONU. Vip. 4. T. 6. S. 181 – 183. [in Ukrainian].