Didenko S. Ju., Golik O. V., Relina L. I., Vechers'ka L. A.
Pages: 41-46.

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Abstract

The purpose. To establish efficient doses of -irradiation of grain of wheat for formation of its amylose-free genotypes, and to create on this basis a line for the further selection of grades of wheat with starch of waxy type. Methods. Biophysical, biochemical, computing-analytical, morphometric. As mutagen factor they used g-irradiation of 60Co in different doses (100; 150 and 200 Gr (Gray)). Lines of wheat irradiated with the purpose of improvement of their adaptive potential to natural settings of Ukraine. Well adapted grade of wheat Kharkivska 30 was irradiated with the purpose of induction of waxy mutation. Results. Effective doses of -irradiation for creation of amylose-free lines of soft spring wheat were established. They selected specific primers to waxy genes in all three genomes of hexaploid wheat. For each pair of primers the comfort temperature of amplification was specified. By means of molecular markers mutant genotypes of wheat with amylose-free type of starch were selected. On the basis of mutant genotypes and local grades they created and transmitted in laboratory of selection of spring wheat 2 lines with waxy starch. Conclusions. The carried out researches have confirmed efficiency of use of the induced mutagenesis for creation of genotypes of soft spring wheat with amylose-free type of starch. On the basis of mutant samples with the changed distillation characteristic of starch new lines of soft spring wheat with a complex of valuable economic attributes — Liutescens 12/16 and Liutescens 30/16 are created. The first line is characterized by early growth, duration of vegetative period — 90–93 days, durability to lodging, average productivity — 2,2–3 t/hectare, protein content — 13,1–14,2%, amylopectin in starch — 99,8–99,9%. At the second line vegetative period — 89–91 days, resistant to lodging, height of plants — 50–52 cm, average productivity — 2,3–3,1 t/hectare, protein content — 13,3–14,4%, amylopectin in starch — 99,8–99,9%.

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Key words: irradiation, amylopectin, genotype, selection, genes, primer, temperature of amplification.

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References

1. Graybosh, R.A. (1998). Waxy wheats: origin, properties and prospect. Food science and technology, 9, 135-142.
2. Abdel-Aal, E.-S. M., Hucl, P., Chibbar, R.N., Han, H.L., & Demeke, T. (2002). Physicochemical and Structural Characteristics of Flours and Starches from Waxy and nonwaxy Wheats. Cereal Chemistry, 79 (3), 458–464.
3. Bhattacharya, M., Erazo-Castrejon, S., Doehlert, D.C., & McMullen, M.S. (2002). Staling of Bread as Affected by Waxy Wheat Flour Blends. Cereal Chemistry, 79 (2), 178–182.
4. Hayakawa K., Tanaka K., Nakamura T., Endo S., & Hoshino T. (2004). End Use of Waxy Wheat Flour in Various Grain-Based Foods. Cereal Chemistry, 81 (5), 666–672.
5. Qin, P., Ma, C., Wu, R., Kong, Z., & Zhang, B. (2009). Effect of waxy wheat flour blends on the quality of fresh and stale bread. Agricultural Sciences in China, 8, 4, 401-409.
6. Kowalski, R.J., Siyuan, Wang, A.M., Joyner, Melito, H., & Ganjyal, G.M.  (2017). Waxy wheat flour as a freeze-thaw stable ingredient through rheological studies. Food and Bioprocess Technology, 10, 7, 1281–1296.
7. Bajaj, R., Singh, N., & Kaur, A. (2019). Effect of native and gelatinized starches from various sources on sponge cake making characteristics of wheat flour. J Food Sci Technol., 56, 2, 1046-1055. doi:10.1007/s13197-019-03632-w.
8. Rybalka, O.I., Polishchuk S.S., & Morhun, B.V. (2018). Novi napriamy v selektsii zernovykh kultur na yakist zerna [New trends in cereal breeding for grain quality]. Bulletin of Agricultural Science, 11(788), 120-133. [In Ukrainian].
9. Alcazar-Alayi, S.C., & Almeida Meirelesi, M.A. (2015). Physicochemical properties, modifications and applications of starches from different botanical sources. Food Science and Technology, 35, 2, 215-236. dx.doi.org/10.1590/1678-457X.6749.
10. Smith, A.M. (2001). The biosynthesis of starch granules. Biomacromolecules, 2, 2, 335-341. http://dx.doi.org/10.1021/bm000133c.PMid:11749190
11. Tester, R.F., Karkalas, J., & Qi, X. (2004). Starch–Composition, fine structure and architecture. Journal of Cereal Science, 39, 2, 151-165. http://dx.doi.org/10.1016/j.jcs.2003.12.001
12. Pérez, S., Bertoft, E. (2010). The molecular structures of starch components and their contribution to the architecture of starch granules: a comprehensive review. Starch/Staerke, 62, 8, 389-420. http://dx.doi.org/10.1002/star.201000013
13. Shu, Q.Y., Forster, B.P., Nakagawa, H. (Eds.) (2012). Plant Mutation Breeding and Biotechnology. CABI: Oxfordshire, UK.
14. Slade, A.J., Fuerstenberg, S.I., Loeffler, D., Steine, M.N., Faccciotti, D. (2005). A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nature biotechnology, 23, 75–81.