07. Mikrobiota of a rumen of sheep at feeding with oil-seed meal from genetically inoculated sunflower
Pomitun I. A., Truskova T. Ju., Rossoha V. I., Grytsyna O. G., Jemel'ianova N. S.
Pages: 34-38.
Full article:
Key words: genetically modified sunflower, shrove, lamb, microbiota, scar contents, amount, activity.
Pages: 34-38.
Full article:
Abstract
Goal. To investigate the influence of genetically modified components in the composition of feed on the microbiota of the sheep's rumen content. Methods. The study was conducted on three analogue groups of 7-month-old lambs, a preparatory period of 8 days. During the month, the animals of the control group received a diet according to their physiological norm and animals of the experimental groups were replaced by a sunflower meal containing 12 and 100% DNA promoter 35S relative to the genomic DNA of soy (respectively, shroot / 12 and shrove / 100). After slaughter of lamb in the content of scar commonly used methods determined the total number of microorganisms, infusoria, mesophilic aerobic and optional anaerobic microorganisms, lactobacillus, microscopic fungi, activity of reductase and cellulosolytic activity, total acidity and concentration of hydrogen ions (pH). Results In animals fed with shrok / 100, compared to those in which the swine / 12 was fed, more general microorganisms were detected (40 times, P> 0.95), less infuzoria (1.75 times, P> 0.95), higher overall acidity (1.2 times, P> 0.95) and higher activity of reductases. Conclusions Reducing the number of infusoria in the lamb's scar tissue, to which the swab / 100 was fed, suggests the presence of factors unfavorable to the development of infusoria.Key words: genetically modified sunflower, shrove, lamb, microbiota, scar contents, amount, activity.
References
- GOCT 10444.12 — 88 Ppodyktbl nyschebble. Metod onpedelehyia dpozhzhej y nlechebblx rpybob.
- GOCT 29184 — 91 Ppodyktbl nyschebble. Metodbl bbliablehyia y onpedelehyia kolychectba baktepyj cemejctba Enterobacteriaceae.
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- Metod z bykopyctobybahhiam arapoboro cepedobyscha Beapd-Papkepa (ISO 6887-1:1999, IDT).
- DCTY ISO 4833:2006 Mikpobioloriia xapchobyx npodyktib ta kopmib dlia tbapyh. Gopyzohtalbhyj metod nidpaxyhky mikpooprahizmib. Texhika nidpaxybahhia kolohij za temnepatypy 30 °C (ISO 4833:2003, IDT).
- DCTY ISO 21571:2008 Ppodykty xapchobi. Metody byiablehhia rehetychho modyfikobahyx oprahizmib i npodyktib z ixhim bmictom. Ekctparybahhia hykleihoboi kycloty (ISO 21571:2005, IDT).
- DCTY ISO 21570:2008 Ppodykty xapchobi. Metody byiablehhia rehetychho modyfikobahyx oprahizmib i npodyktib z ixhim bmictom. Kilbkichi metody ha ochobi ahalizybahhia hykleihoboi kycloty (ISO 21570:2005, IDT).
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