06. Influence of phenolic matters upon efficiency of use of cultivars Rosa damascena Mill. as crop in vitro

https://doi.org/10.31073/agrovisnyk201602-06
Olijnyk O. O., Kliuvadenko A. A., Lihanov A. F., Mel'nychuk M. D.
Pages: 28-31.

Full article: 
Abstract
The purpose. To study links between strain-specificity of polyphenolic bonds and recovery processes of explants of rose-tree in conditions in vitro. Methods. Culture of tissues and organs of plants in vitro, thin-layer chromatography, spectophotometry. Results. It is fixed that the greatest content of phenolic bonds is characteristic for cultivars of rose-tree Lada and Raduga, and the least one — for cultivar Lan. It is determined that the heightened content of phenolic bonds and their accumulation in tissues and nutrient medium negatively influences sterilization and recovery processes of primary explants in vitro. Conclusions. Quality content and amount of simple phenols and condensed tannins in leaves of rose-tree of essential oilbearing cultivars Lada, Lan, Raduga is the marker of potential ability of scions of cultivars of essential oilbearing rose-tree to neogenesis. In conditions of presence of phenolic bonds in leaves of primary explants in density below 60 mg/g of crude mass efficiency of use of plants as crop in vitro is increased.


Key words: essential oilbearing rose-tree, explants, nutrient medium, phenolic bonds, crop in vitro.



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