Starodub L. F.
Pages: 44-51.

Full article: 

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Abstract

The purpose. Cytogenetic analysis of grey Ukrainian cattle and comparison of stability of its karyotype with retrospective data concerning animal breeds of different directions of productivity. Methods. Cytogenetic — analysis of metaphase plates of cells of peripheral blood of cattle; microkernel test, statistical. Results. The share of cells with aneuploidy at animals of grey Ukrainian breed made 1,2%; average arithmetical amount of aneuploidic cells at animals of breeds of milk direction of productivity — 2,4%, that twice exceeded this index at grey Ukrainian cattle. At animals of breeds of the combined direction aneuploidy made 2%, at animals of breeds of meat direction of productivity — 10,6% with statistically reliable variance (P>0,99). Variability on indexes of somatic polyploidy is not detected in cows of grey Ukrainian breed, share of 2-nuclear lymphocytes — 2,2‰, that twice below, and mitotic activity of peripheral blood lymphocytes — 2,8‰, that is authentically below (P>0,99) in comparison with the animals specified above. Conclusions. The karyotype of probed cows of grey Ukrainian breed was characterized by the heightened stability in comparison with animals of breeds of milk, combined and meat directions of productivity at spontaneous mutagenesis.

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Key words: grey Ukrainian breed, aneuploidy, polyploidy, chromosome breaks, microkernel test, 2-kernel lymphocyte, mitotic index.

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References

1. Mekhanizmy formuvannia prykhovanoi henetychnoi minlyvosti u henofondi velykoi rohatoi khudoby/V.S. Konovalov, I.V. Huziev, K.V. Kopylov ta in.//Nauk. visn. nats. un-tu bioresursiv i pryrodokorystuvannia Ukrainy. — 2011. — № 160. — S. 311–317.
2. Kozyr V.S. Sira ukrainska poroda: natsionalne nadbannia na mezhi znyknennia/V.S. Kozyr//Ahrobiznes sohodni. — 2015. — № 22 (317). — S. 3.
3. Tsytohenetychnyi analiz velykoi rohatoi khudoby za khronichnoho nyzkodozovoho oprominennia ta infikuvannia orhanizmu virusom leikozu/S.O. Kostenko, P.P. Dzhus, L.F. Starodub ta in.//Ahroekoloh. zhurn. — 2011. — № 3. — S. 129–133.
4. Shelov A.V. Metodyka pryhotuvannia metafaznykh khromosom limfotsytiv peryferiinoi krovi tvaryn/A.V. Shelov, V.V. Dzitsiuk//Metodyky naukovykh doslidzhen iz selektsii, henetyky ta biotekhnolohii u tvarynnytstvi: nauk. zb. — K., 2005. — S. 210–213.
5. Vyznachennia henetychnykh anomalii u velykoi rohatoi khudoby: metodychni rekomendatsii/M.I. Bashchenko, K.V. Kopylov, M.L. Dobrianska ta in. — Chubynske, 2011. — 36 s.
6. Chebotarev A.N. Zakonomernosty khromosomnoi yzmenchyvosty somatycheskykh kletok cheloveka/A.N. Chebotarev//Vestn. RAMN. — 2001.— T. 37, № 10. — S. 64–69.
7. Hlazko T.T. Mikroiadernyi test u velykoi rohatoi khudoby/T.T. Hlazko//Visn. ahrar. nauky. — 2001. — № 9. — S. 45–48.
8. Kostenko S. Tsytohenetycheskyi kontrol molochnoho y miasnoho krupnoho rohatoho skota/S. Kostenko, L. Starodub//Institutul stiintifico-practic de biotehnologii in zootehnie si medicinà veterinara. — Maximovca, 2011. — S. 417–422.
9. Relationship between genotoxicity biomarkers in somatic and germ cells: findings from a biomonitoring studu/L. Migliore, R. Colognato, A. Naccarati et al.// Mutagenesis. — 2006. — № 21 (2). — P. 149–152.
10. Kovaleva O.A. Tsytohenetycheskye anomalyy v somatycheskykh kletkakh mlekopytaiushchykh/O.A. Kovaleva//Tsytolohyia y henetyka. — 2008. — T. 1. — S. 58–72.
11. Rukovodstvo po kratkosrochnym testam dlia vyiavlenyia mutahennykh y kantserohennykh khymycheskykh veshchestv//Sovmestnoe yzdanye Prohrammy OON po okruzhaiushchei srede, Mezhdunarodnoi orhanyzatsyy truda y Vsemyrnoi orhanyzatsyy zdravookhranenyia. — Zheneva: Vsemyrnaia orhanyzatsyia zdravokhranenyia, 1989. — 51 s.