Mandygra M. S., Dolets'kyj S. P., Kutsan O. T., Shevtsova G. M., Roman'ko M. Je., Orobchenko O. L., Gerilovych I. O.
Pages: 32-38.

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Abstract

The purpose. To study influence of trans-gene soya of line MON 89788 on an organism of laboratory animals. Methods. Toxic-hygienic researches were spent on the basis of department of toxicology, safety and quality of agricultural production of NSC «IEKVM» in view of main principles of bioethics. Experiment in vivo has been put on males of white mice (90 days). Before the beginning of the experiment animals were divided by the principle of analogues into 3 groups (20 animals in each group) and during 10 days maintained in the leveling period. In the further mice of control group received basic diet (BD) — grain mix without soya. Mice of the I-st group received BD, in which 30% of weight of grain was replaced with crushed and thermally processed (120°C, 30 min) soya of average ripeness without genic modification; mice in the II-nd group — received BD, in which 30% of weight of components was replaced with crushed and thermally processed (120°C, 30 min) soya of line MON 89788. During experiment with mice they carried out clinical supervision (considered appetite, consumption of water, reaction on external irritation, behaviour, etc.) . On the 21-st, 45-th and 90-th day from the beginning of experiment they carried out mice euthanizing by decapitation with the use of chloroformic narcosis (5 animals from each group). They also selected blood samples for carrying out the further biochemical researches and paid attention to presence of pathological-and-anatomic changes. Results. Long-term receipt (during 90 days), unlike short-term (during 21, 45 days), in an organism of laboratory animals of soya of line MON 89788 led to negative results. Pathological-and-anatomic changes in liver and gastroenteric path were revealed. By biochemical markers (increase of activity alaninaminotranspherase in all periods of research, increase of concentration of gama-globulins, for 21 day of experiment moderate growth of beta-globulins (P≤0,05), authentic increase of content of general protein for 90-th day) was fixed heavier infringement of functional state of liver at animals who received modified soya, rather than traditional one. Conclusions. It is expedient to continue researches of transgenic soya with the use of other kinds of laboratory and agricultural animals.

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Key words: GM-soya of line MON 89788, white mice, pathological-and-anatomic changes, functional state of liver, blood plasma.

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