Roik M. V., Kotsar M. O.
Pages: 39-44.

Full article: 

---

Abstract

The purpose. To determine dynamics of anatomical-morphological parameters of miscanthus sprouts got from culture in vitro at cultivation the open ground. Methods. Technique of clonal microduplication of miscanthus (2013) is used, biometric, statistical. Results. Survival factor of plants of miscanthus is established for different genotypes from culture in vitro in conditions of open ground, as well as dynamics of anatomical-morphological parameters of miscanthus of different genotypes got from culture in vitro in conditions of cultivation in conditions in vivo within 3 years of vegetation without entering mineral fertilizers on light grey wood subacidic soil (pH=5,4) at regular irrigation. The first anatomical-morphological parameters at plants of miscanthus were determined in 3 months of vegetation after planting. They estimated key parameters of growth and development of plants of miscanthus in system of economic-valuable attributes — components of efficiency. For three years of cultivation of miscanthus they observed increase in amount of shoots on the average from 16 up to 41 pieces, in height of plants — from 92,8 up to 181,8 cm, in area of sheet surface — from 31,8 up to 93,5 cm2. Genotypes of miscanthus with intensive increase of structural elements of biomass are allocated. Efficiency of methods of biotechnology for getting qualitative planting material of miscanthus is estimated. Conclusions. Survival factor for sprouts of miscanthus of different genotypes got from culture in vitro in conditions of field experiment varies within the limits of 79 – 98%. For 3 years of cultivation of plants of miscanthus of six genotypes from collection of the institute of various origin got from culture in vitro form on the average 30 – 49 shoots in height of 100,2 – 241,8 cm. The greatest intensive increase of amount of shoots, height of plants, amount of leaves and area of sheet surface on test field “Batyieva Hora” of the institute were characterized for genotypes of kinds M. giganteus, M. sinensis, M. sinensis Early. Use of methods of biotechnology enables to receive qualitative planting material of miscanthus for growing biopower plantations in conditions of Forest-steppe of Ukraine.

---

Key words: miscanthus, in vitro, sprouts, anatomical-morphological parameters, survival factor.

---

References

1. Roik M.V., Yaholnyk O.O. (2014). Bioenerhetyka yak nauka y haluz ekonomiky: istoriia, kontseptsiia, periodyzatsiia (etapy) rozvytku. [Bioenergy as a science and branch of economy: history, concept, periodization (stages) of development]. Bioenerhetyka. №1 (3). P. 7-11. [in Ukrainian].
2. McLaughlin S.B., Walsh M.E. (1998) Evaluating environmental consequences of producing herbaceous crops for bioenergy. Biomass Bioenergy. Vol. 14. P. 317-324.
3. Khanna M., Dhungana B., Clifton-Brown J. (2008) Costs of producing miscanthus and switchgrass for bioenergy in Illinois. Biomass and Bioenergy. Vol. 32, Issue 6. P. 482-493.
4. Los L.V., Zinchenko V.O., Zhaivoronovskyi V.R. (2011). Vyroshchuvannia i hazyfikatsiia biopalyv — efektyvnyi shliakh vyrishennia «enerhetychnykh» i ekolohichnykh problem na prykladi miskantusa hihanteusa. [Cultivation and gasification of biofuels are an effective way to solve "energy" and environmental problems on the example of the Miskanthus giantheus]. Visnyk ZhNAEU. № 2. P. 46-57. [in Ukrainian].
5. Humentyk M.Ya. (2010). Perspektyvy vyroshchuvannia bahatorichnykh zlakovykh kultur dlia vyrobnytstva biopalyva. [Prospects for growing perennial crops for production biofuels]. Tsukrovi buriaky. № 4. P. 21-22. [in Ukrainian].
6. Kim H.S., Zhang G., Juvik J.A., Widholm J.M. (2010). Miscanthus × giganteus plant regeneration: effect of callus types, ages and culture methods on regeneration competence. GCB Bioenergy. Vol. 2. P. 192-200.
7. Roik M.V., Kornieieva M.O. (2015) Napriamy, metody ta stratehiia rozvytku selektsii. [Areas, methods and strategy of breeding development]. Tsukrovi buriaky. № 6. P. 7-9. [in Ukrainian].
8. Govil S., Gupta S.C. (1997). Commercialization of plant tissue culture in India. Plant cell, tissue and organ culture. Vol. 51. P. 65-73.
9. Bekh N.S., Kotsar M.O. (2016) Klonalne mikrorozmnozhennia miskantusu, yak sposib otrymannia posadkovoho materialu. [Clonal Microcropping of the Miscanthus, as a way of obtaining a landing material]. Bioenerhetyka. № 1 (7). P. 26-28. [in Ukrainian].
10. Dospekhov B.A. (1985) Metodyka polevoho oputa. [Field experience]. Moskva: Ahropromyzdat,. 351 p. [in Russian].