05. Development of molecular-biological system to identify RNA-4 of Ukrainian isolate of the virus of necrotizing yellowing of veins of beet

Grynchuk K. V., Antipov I. O.
Pages: 24-26.

Full article: 
Abstract
Goal. To develop a system for the identification of RNA-4 Ukrainian isolates of the necrotic yellowing virus of beetroot veins (VNPZHB) on the basis of the polymerase chain reaction. Methods. Molecular- biological. Results A bioinformative analysis of nucleotide sequences of RNK-4 isolates of VNPZHB was carried out. Conservative sequences of the gene encoding protein R31 VNPZHB were established, and primer design was developed for the identification of RNA-4. The PLR-system of identification of RNA-4 has been optimized for the temperature indices of the annealing of primers. Conclusions The developed diagnostic system allows timely identification of VNPZHB to prevent its spread.

Key words: virus necrotic yellowing of beetroot veins, protein, RNA.

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