Assessment of resistance of plants of Nicotiana tabacum L. and Nicotiana rustica L. to necrotic and ordinary strains of Y-virus of potato

Abstract

The purpose. To study resistance of grades of tobacco Nicotiana tabacum and makhorka Nicotiana rustica in conditions of artificial becoming infected with necrotic and ordinary isolates of Potato virus Y (PVY). Methods. Molecular-and-biological properties of isolates of Potato virus Y were determined using methods of transmission electronic microscopy, multiplex inverse polymerase chain reaction and ELISA. Biological properties of isolates were studied using method of plant-indicators. Resistance of plants of cultivars of tobacco and makhorka against isolates of PVY were studied using artificial inoculation with subsequent visual detection of symptoms of becoming infected and detection of viruses by method of transmission electronic microscopy and ELISA. Results. Probes of excreted from plants of potato isolates of Potato virus Y had shown that they belonged to different strain groups as to response to their infection contamination which explicated at plant-indicators. Multiplex OT-PCR had confirmed presence of Potato virus Y. and absence of other viruses infecting potato. They had detected different response of plants of tobacco of grades Samsun 155 and Sobalchskyi 34/40 against ordinary and necrotic isolates. With methods of transmission electronic microscopy and ELISA they had confirmed accumulation of this virus. Conclusions. Plants of grades Samsun 155 and Sobalchskyi 34/40 in comparison to ordinary isolate had manifested response of tolerance. These grades were non-resistant against necrotic isolate. Absence of symptoms at plants of makhorka and tobacco of grade Sobalchskyi 193 could testify to tolerance response and availability of resistance against these isolates.