Anti-virus properties of titanium nano-particles

Abstract

Goal. To study the influence of antiviral properties of titanium (Ti) nano-particles (NP) on the reproduction of plant and animal viruses. Methods. Analysis of literature sources, virological, serological, instrumental, and statistical. Results. It was found that the studied preparations contain spherical TiNP with sizes from 2 to 81 nm. Experiments were performed to determine the antiviral activity of TiNP against the strain Teschovirus A of the first serotype (PTV1) Dniprovskyi 34, and the necrotic strain Potato virus Y (PVY) ML1 by prophylactic, therapeutic, and virucidal schemes. In the transplanted culture of swine embryonic kidney cells (SEKC), it was found that TiNP showed little antiviral activity, reducing the virus titer in both schemes by only 0.50 lg TCD50/cm3. However, according to the virucidal scheme, it was found that TiNP at 24 h exposure at the maximum allowable concentration (for SEKC culture — 12.5 μg/cm3) showed high virucidal activity against virus strain PTV1 Dniprovskyi 34, significantly reducing its infectious titer in SEKC cell culture by 4.46 lg TCD50/cm3. It was found that TiNP does not have the antiviral activity to PVY in prophylactic and treatment regimens. As a result of the study of the antiviral activity of TiNP according to the virucidal scheme on tobacco plants, it was found that TiNP has high antiviral activity against PVY. Conclusions. TiNP were found to have high antiviral activity against Teschovirus A strain of the first serotype of Dniprovskyi 34, and necrotic strain of PVY ML1, which belongs to the genus Potyvirus of the Potyviridae family. The obtained results convincingly testify to the prospects of development of effective antiviral nano-preparations and the need for a comprehensive study of the effect of TiNP not only on pathogens but also on the body of animals and crops.