Bactericidal efficiency, phenolic coefficient, and protein index of disinfectant Biolaid against Escherichia coli

Abstract

Goal. To study the bactericidal activity, and bacteriostatic properties, to select the most optimal bactericidal effective concentrations of the new disinfectant (DI) Biolaid for its effect against the test culture of Escherichia coli ATSS 25922 in different time exposures, and to determine its phenolic coefficient and protein index. Methods. Microscopic, cultural, biochemical, statistical. Results. The results of the research on the properties of the new DI Biolaid are presented, the main components of which are components that after a certain time break down into safe water, oxygen, and lactic acid. The bactericidal efficiency was experimentally investigated of the working solutions 0.1; 0.2; 0.25; 0.5; 1.5; 4, and 10% concentrations and optimal concentrations of Biolaid working solutions were selected according to their influence during 10, 20, 30, and 60 minutes. A test was conducted to identify the bacteriostatic properties of DI Biolaid, and the analysis of the results of which proved their absence. The results of experiments on the study of the phenolic coefficient are presented, which indicate the greater efficiency of DI Biolaid compared to the standard phenol solution. The indicators of the protein index of DI Biolaid were established, which indicated a decrease in the effectiveness of this agent due to protein contamination. Conclusions. It was found that 0.25% and higher concentrations of the working solutions of the new DI Biolaid provided complete neutralization of the test culture E. coli ATSS 25922 after exposure for 30 minutes, and 0.1% and higher concentrations after exposure for 60 minutes without the manifestation of bacteriostatic properties. The phenol coefficient of the new DI Biolaid was 7.95 times higher than the bactericidal activity of the standard phenol solutions. Protein contamination can reduce the bactericidal efficiency of the new DI Biolaid by 2.07 times.